The karyological features of Akis subtricostata was determined for the first time with conventional and silver nitrate staining. The diploid number 2n=16 and meioformula 7+neoXY represents a deviation from the modal karyotype of Coleoptera. The pericentromeric heterochromatin was detected with both Giemsa and silver nitrate staining. In addition to determining a single possible NOR on prophase I nuclei, AgNO3 revealed that several telomeric regions of mitotic metaphase chromosomes were slightly more argyrophilic.

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