Xin Cui, Jiachen Zhang, Yitong Liu, Wen Shao, Yawen Tang, Hanjun Sun

A Portable Nanozyme‐Strip Fabricated by Ru‐ZIF‐8 with Dual Nanozymatic Activity for Detection of Acetylcholine

  • Materials Chemistry
  • Energy Engineering and Power Technology
  • Renewable Energy, Sustainability and the Environment
  • Biomaterials
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AbstractIn the present study, the incorporation of Ru into the framework of ZIF‐8 resulted in that a Ru‐ZIF‐8 nanozyme with both esterase‐like activity and peroxidase‐like activity. Owing to the dual nanozymatic activities of Ru‐ZIF‐8 nanozyme, the acetylcholine (ACh) detection process utilized only one natural enzyme (choline oxidase), which greatly simplified the detection procedure. In the detection process, nanozyme Ru‐ZIF‐8 showed esterase‐like activity to catalyze the hydrolysis of ACh to choline. Then the generated choline was oxidized by choline oxidase to generate H2O2. Finally, Ru‐ZIF‐8 with peroxidase‐like activity was used to catalyze H2O2 to oxidize 2,2′‐azino‐bis (3‐ethylbenzothiazo‐line‐6‐sulfonic acid) ammonium salt with a color change. As a result, the linear range for ACh was from 0.01 μM‐22 μM with the detection limit (DL) of 5.5 nM. In addition, Ru‐ZIF‐8 was utilized to fabricate a portable detection test strip. Our work provided a simple, portable, low‐cost, highly sensitive and selective assay for the detection of ACh, which paved the way for the development of multi enzyme cascade assays.

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