Advanced Sequencing Techniques to Map RNA Methylation

Abstract

RNA methylation is a crucial epigenetic modification widely present in RNA molecules, and has been demonstrated to play significant roles in diverse biological processes. Advances in detection and sequencing technologies have facilitated the identification of RNA modification‐related regulatory proteins and their corresponding biological functions. In this paper, we provide a brief overview of several RNA methylation, including N⁶‐methyladenosine(m⁶A), 5‐methylcytidine(m⁵C), N¹‐methyladenosine(m¹A), N⁷‐methylguanosine(m⁷G) and N⁶, 2’‐O‐dimethyladenosine(m⁶Am), about their regulatory proteins, distribution patterns and biological functions, and mainly outline the advantages and limitations of the representative sequencing techniques. Finally, we discuss the technological challenges and future perspectives in RNA transcriptomic field.