Ameliorative Effects of Dimethyl Sulphoxideand Fisetin on Oxidative Stress Biomakers in Lame Horses

Lame horses are more susceptible to oxidative stress, an imbalance in the oxidant/antioxidant system in cells. The aim of this study is to evaluate the effects of Dimethyl Sulphoxide and fisetin in combating oxidative stress, and biomarkers in lame horses. Dimethyl Sulphoxide and fisetin were administered singly, in half doses and in combination to lame horses, their activities on antioxidant enzymes (catalase, glutathione peroxidase, and superoxide dismutase) and malondialdehyde were observed. Twenty-five lame horses, numbered and selected using purposive simple random sampling without replacement, were distributed into five groups (A-D) of five horses each showing clinical signs of lameness (Graded from 0 to 2). Dimethyl Sulphoxide (1 mg/kg) was administered through intravenous injection for each horse once per week while fisetin was administered per os at 10 mg/kg and given daily for 60 days. The result showed that there was a significant reduction in MDA concentration from 737.22 ± 0.63 nmol/mg to 216.0.76 nmols/mg, and SOD activity was increased from 11.12± 0.07 to 12.10 ± 0.20, While CAT activity was increased significantly from 39.88± 0.48 IU/L to 62.44 ± 0.58 IU/L. GPx was reduced from 17.62 ± 0.38 IU/L to 17.46 ± 1.44 IU/L. There was a significant decline in the oxidative stress biomarker in lame horses with administration of dimethyl sulphoxide and fisetin.