Changes in land-use–land-cover (LULC) affect the water balance of a region by influencing the water yield (WY) along with variations in rainfall and evapotranspiration (ET). Remote sensing satellite imagery offers a comprehensive spatiotemporal distribution of LULC to analyse changes in WY over a large area. Hence, this study mapped and analyse successive changes in LULC and WY between 2000 and 2015 in the Johor River Basin (JRB) by specifically comparing satellite-based and in-situ-derived WY and characterising changes in WY in relation to LULC change magnitudes within watersheds. The WY was calculated using the water balance equation, which determines the WY from the equilibrium of precipitation minus ET. The precipitation and ET information were derived from the Tropical Rainfall Measuring Mission (TRMM) and moderate-resolution imaging spectroradiometer (MODIS) satellite data, respectively. The LULC maps were extracted from Landsat-Enhanced Thematic Mapper Plus (ETM+) and Landsat Operational Land Imager (OLI). The results demonstrate a good agreement between satellite-based derived quantities and in situ measurements, with an average bias of ±20.04 mm and ±43 mm for precipitation and ET, respectively. LULC changes between 2000 and 2015 indicated an increase in agriculture land other than oil palm to 11.07%, reduction in forest to 32.15%, increase in oil palm to 11.88%, and increase in urban land to 9.82%, resulting in an increase of 15.76% WY. The finding can serve as a critical initiative for satellite-based WY and LULC changes to achieve targets 6.1 and 6.2 of the United Nations Sustainable Development Goal (UNSDG) 6.

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