Metin ÇALIŞKAN, Gulsen BAYRAK, Serçin ÖZLEM ÇALIŞKAN

Anti-leukemic Effect of Malachite green-mediated Photodynamic Therapy by inducing ER stress in HL60 cells.

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
Save to CiteDrive Show PDF

Aim: In this study, we aimed to examine of anti-leukemic effect and the relationship with endoplasmic reticulum (ER) stress of malachite green-mediated photodynamic therapy (PDT) in HL60 acute myeloid leukemia cells. Material and Methods: The cells were incubated with different concentrations (3.125, 1.56, 0.39, 0.195, 0.0975, 0. 04875 μM) of malachite green for one hour, were exposed to delivered an irradiance of 0.47 mW/cm2 and a fluence of 0.84 J/cm2 at 30 minutes. Also, HL60 cells were exposed for PDT with light only and both in the presence or absence of malachite green. MTT was used to evaluate cell viability and immunocytochemical staining was used to determine ER stress markers Glucose-regulated protein 78 (GRP78) and Protein Kinase R-like ER Kinase (PERK). Results: Cell viability was significantly decreased in the treatment group (combination of malachite green and light) compared to the malachite, light and control groups. Moreover, immunocytochemical staining scoring showed that GRP78 and PERK were significantly upregulated in the treatment group compared to other groups. Conclusion: The results presented in this study indicate that ER stress may contribute to the cytotoxicity occurring in HL60 cancer cells after malachite green-mediated PDT. Future studies will be crucial in shedding light on the molecular mechanisms underlying ER stress that may occur after PDT. These findings lay the foundation for further investigations in this area.

Need a simple solution for managing your BibTeX entries? Explore CiteDrive!

  • Web-based, modern reference management
  • Collaborate and share with fellow researchers
  • Integration with Overleaf
  • Comprehensive BibTeX/BibLaTeX support
  • Save articles and websites directly from your browser
  • Search for new articles from a database of tens of millions of references
Try out CiteDrive

More from our Archive