Basic histology of hard tissues of teeth using ESEM of ground section and digital drawings
Anam Khan, Sandhya TamgadgeObjectives:
While the histological features of teeth are well-established, there is a continuing need for innovative approaches to visualize and communicate these complex structures effectively, particularly for educational purposes and as a foundation for future research. The objectives of this study were as follows: (1) To integrate environmental scanning electron microscopy (ESEM) imaging with digital drawings to create a novel visual representation of dental hard tissues. (2) To explore the potential of readily available technology (iPad and pencil) for creating accurate integrated histological illustrations of all features together, based on advanced microscopy data. (3) To develop a methodological approach that bridges the gap between microscopic imaging and schematic representations of dental microstructure.
Material and Methods:
A single tooth ground section was examined using ESEM. Additionally comprehensive digital drawings of all hard tissues were created using an iPad and pencil. All the histological features of tooth were also drawn in a single diagram. This combination of techniques allowed for detailed visualization of enamel, dentin, and cementum structures.
Results:
The study produced a series of high-resolution ESEM images coupled with corresponding digital illustrations, providing a unique visual resource for dental histology. While not presenting new ultrastructural findings, the study demonstrates a novel approach to representing known structures.
Conclusion:
This study, while descriptive in nature, introduces an innovative method for visualizing and illustrating dental histology. The integration of ESEM with digital drawing techniques offers a new perspective on dental microstructure, with potential applications in dental education and as a foundation for future research methodologies.