Characterization and Identification of Oleaginous Microalgae Isolated from Fresh Water for Biodiesel Production
Radhika Jaithaliya, Divya Jain, Dipjyoti Chakraborty, Kakoli DuttIntroduction:
Developing sustainable biodiesel production relies on investigating local microalgal populations to detect neutral lipid accumulation via high throughput screening. Method: This study evaluates the efficacy of using various isolation strategies for maximizing microalgal strain collection from low-abundance water samples.
Result:
The study resulted in the isolation of twenty-five algal strains, of which 3 oleaginous strains were identified as Chlorococcum aquaticum BB607, Chlorococcum sp. BB601 and Spongiosarcinopsis limneus BG607 were selected. Isolate C. aquaticum BB607 exhibited substantial lipid content of 456.45 ± 2.40 μg/mL, lipid productivity of 38.04 ± 0.20 μg/mL/day, biomass yield of 4.23 ± 0.06 mg/mL and maximum percentage C16-C18 fatty acid profile compared to the other two isolates.
Conclusion:
Further, the FAMEs produced from this isolate exhibited high CN (60.384), low iodine value (97.33 g I2/100 g) and negative cold filter plugging point (- 2.28°C). This demonstrates the potential of C. aquaticum strain BB607 as a feedstock biodiesel production.