Construction and analysis of a joint diagnostic model of machine learning for cryptorchidism based on single‐cell sequencing
Yuehua Chen, Xiaomeng Zhou, Linghua Ji, Jun Zhao, Hua Xian, Yunzhao Xu, Ziheng Wang, Wenliang Ge- Health, Toxicology and Mutagenesis
- Developmental Biology
- Toxicology
- Embryology
- Pediatrics, Perinatology and Child Health
Abstract
Background
Cryptorchidism is a condition in which one or both of a baby's testicles do not fully descend into the bottom of the scrotum. Newborns with cryptorchidism are at increased risk of developing infertility later in life. The aim of this study was to develop a novel diagnostic model for cryptorchidism and to identify new biomarkers associated with cryptorchidism.
Methods
The study data were obtained from RNA sequencing data of cryptorchid patients from Nantong University Hospital and the Gene Expression Omnibus (GEO) database. Differential expression analysis was used to obtain differentially expressed genes (DEGs) between the control and cryptorchid groups. These DEGs were analyzed for their functions by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment using GSEA software. Random Forest algorithm was used to screen central genes based on these DEGs. Neuralnet software package was used to develop artificial neural network models. Based on clinical data, receiver operating characteristic (ROC) was used to validate the models. Single‐cell sequencing analysis was used for the pathogenesis of cryptorchidism.
Results
We obtained a total of 525 important DEGs related to cryptorchidism, which are mainly associated with biological functions such as supramolecular complexes and microtubule cytoskeleton. Random forest approach screening obtained eight hub genes. A neural network based on the hub genes showed a 100% success rate of the model. Finally, single‐cell sequencing analysis validated the hub genes.
Conclusion
We developed a novel diagnostic model for cryptorchidism using artificial neural networks and validated its utility as an effective diagnostic tool.