Contribution of crossing over to genetic variance in maize and wheat populations

Abstract

Crossing over breaks linkages and leads to a wider array of allele combinations. My objective was to assess the contribution of crossing over to genetic variance (V_G) in maize (Zea mays L.) and wheat (Triticum aestivum L.). The contribution of crossing over to V_G (denoted by P_CO) was assessed by calculating V_G without crossing over from the sums of marker effects on each chromosome and by estimating V_G with crossing over from simulated doubled haploids that arise from meiosis. For maize yield, crossing over had positive contributions of P_CO = 7% and 16% in two populations but it strongly decreased V_G (P_CO = −74% to −25%) in five other populations. The mean P_CO was negative for moisture, test weight, plant height, and ear height. In wheat, the P_CO values were all negative for five traits in the Louise/Penawawa population but were all positive for three traits in the Seri/Babax population. Negative P_CO values were attributed to large differences between the sum of allelic effects on a homolog inherited from one parent and the sum of effects on the homolog from the other parent. Although crossing over most often decreased V_G, the best simulated line (out of 10,000) with crossing over was usually superior to the best line without crossing over. Breeding progress will therefore continue to rely on finding individuals with increasingly rare, favorable crossovers amidst individuals with crossovers that are mostly unfavorable.