DOI: 10.1002/cbic.202300731 ISSN: 1439-4227
De novo Semi‐Synthetic Platform for Monitoring Protein degradation in Live Cells
Mahdi Hasan, Deepanjan Panda, Guy Mann, Ashraf Brik- Organic Chemistry
- Molecular Biology
- Molecular Medicine
- Biochemistry
Abstract
We designed a platform for monitoring the degradation of exogenous proteins in live cells. We engineered a semi‐synthetic platform, which consists of Enhanced Green Fluorescent Protein tagged with SpyCatcher to enable its conjugation to a SpyTag peptide bearing a Von Hippel–Lindau E3 ligand, which was delivered to live cells to promote its degradation. This platform lays the ground for studying the degradation of endogenous proteins equipped with SpyTag and for tracking the degradation of post‐translationally modified proteins in live cells.