Effect of non‐surgical periodontal therapy on salivary histone deacetylases expression: A prospective clinical study
Andrew Liaw, Chun Liu, Mark Bartold, Sašo Ivanovski, Pingping Han- Periodontics
Abstract
Aim
To evaluate the effect of non‐surgical periodontal therapy (NSPT) on salivary histone deacetylases (HDACs) gene expression in patients with Stage III–IV periodontitis at baseline and at 3 and 6 months post NSPT treatment.
Materials and Methods
Twenty patients completed the study. Periodontitis (as well as the corresponding staging and grading) was diagnosed according to the 2017 World Workshop Classification. Clinical measures were recorded and whole unstimulated saliva was collected at baseline and at 3 and 6 months after NSPT. The expression of 11 HDACs was determined using reverse‐transcription PCR, and the respective changes over time were evaluated.
Results
Six months after NSPT, significant improvements in all clinical periodontal parameters were observed, concomitant with significant up‐regulation of HDAC2, 4, 6, 8, 9 and 11 expressions. Subgroup analyses of non‐responders and responders revealed no significant differences in HDACs mRNA expression between groups at any time point.
Conclusions
This prospective clinical study identified longitudinal changes in salivary HDACs expression in response to NSPT, which provides new insights into the epigenetic mechanisms underlying the pathobiology of periodontitis and creates avenues for the discovery of novel biomarkers.