Fabrication and Evaluation of Hyaluronic Acid Coated Albumin Nanoparticles for Delivery of Gemcitabine
Shweta Paroha, Ravindra Dhar Dubey, Juhi Verma, Vikas Jain, Saleem Akbar, Ashwini Kumar Mishra, S.L. Neha, Laxmi Rani, Aman Kumar Mahto, Parvat Kumar Sahoo, Rikeshwer Prasad DewanganAbstract:
Gemcitabine (Gem) is a well-known antineoplastic drug used for several solid tumors. The clinical application of gem is hampered owing to non-selectivity, short half-life, and drug resistance, which necessitate the development of a suitable novel formulation that can selectively target cancer sites. In the present work, Gem-loaded bovine serum albumin nanoparticles (Gem-BSANPs) have been prepared and coated with hyaluronic acid (HA-Gem-BSANPs). Particle size, zeta potential, TEM, and DSC analysis characterized the developed NPs. The mean particle size, PDI, and zeta potentials were observed to be 120.9 ± 5.87 vs 144.7 ± 5.67 and 28.66 ± 1.10 vs -45.72 ± 3.24, for Gem-BSANPs and HA-Gem-BSANPs, respectively. Interestingly, HA-coated Gem-BSANPs were found higher cytotoxic against A549 cell lines with better killing kinetics and mitochondrial membrane loss due to overexpression of CD44. The present work demonstrated that HA-Gem-BSANPs could be a potential strategy to improve the therapeutic efficacy of gem by selectively targeting to the tumor site.