Human skin biofilm model: translational impact on swabbing and debridement

doi:10.12968/jowc.2023.32.7.446

Michael Dietrich, Manuela Besser, Eike S Debus, Ralf Smeets, Ewa K Stuermer

Human skin biofilm model: translational impact on swabbing and debridement

Nursing (miscellaneous)
Fundamentals and skills

Objective: Wound biofilms are one of the greatest challenges in the therapy of hard-to-heal (chronic) wounds, as potent antimicrobial substances fail to eradicate bacteria within short incubation periods. Preclinical investigations using novel model systems that closely mimic the human wound environment and wound biofilm are required to identify new and effective therapeutic options. This study aims to identify bacterial colonisation patterns that are relevant for diagnosis and therapy. Method: In this study, a recently established human plasma biofilm model (hpBIOM) was incorporated into a wound within human dermal resectates after abdominoplasty. The interaction of the biofilm-forming bacteria meticillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa with the skin cells was investigated. Possible effects on wound healing processes in correlation with the persistence of the biofilm in the wound environment were analysed in patients with leg ulcers of different aetiologies and biofilm burden. Results: Using haematoxylin and eosin staining, species-dependent infiltration modes of the bacteria into the wound tissue were determined for the pathogens MRSA and Pseudomonas aeruginosa. The spreading behaviour correlated with clinical observations of the spatial distributions of the bacteria. In particular, the clinically prominent Pseudomonas aeruginosa-specific distension of the wound margin was identified as epidermolysis due to persistent infiltration. Conclusion: The hpBIOM applied in this study represents a potential tool for preclinical analyses dealing with approval processes for new antimicrobial applications. In terms of clinical practice, a microbiological swabbing technique including the wound margin should be routinely applied to prevent wound exacerbation.

Need a simple solution for managing your BibTeX entries? Explore CiteDrive!

Web-based, modern reference management
Collaborate and share with fellow researchers
Integration with Overleaf
Comprehensive BibTeX/BibLaTeX support
Save articles and websites directly from your browser
Search for new articles from a database of tens of millions of references

Try out CiteDrive

Human skin biofilm model: translational impact on swabbing and debridement

Need a simple solution for managing your BibTeX entries? Explore CiteDrive!

More from our Archive

Characterizing Persons With HIV/HCV Coinfection Who Remain Untreated for Hepatitis C at Four HIV Clinics in Connecticut (CT): Role of Multiple Overlapping Barriers at the Individual and Clinic System Levels

Creating a Longitudinal HCV Care Cascade for Persons With HIV/HCV Coinfection in Selected HIV Clinics Using Data to Care Methods

Future Directions for HIV/HCV Care: Lessons Learned From Local Evaluation Projects in Texas and Connecticut and Implications for Practice and Health Promotion

Human skin biofilm model: translational impact on swabbing and debridement

Advancing Inclusion of Transgender and Gender-Diverse Identities in Clinical Education: A Toolkit for Clinical Educators

Relaxation Methods to Reduce Occupational Stress

Tele-Assessment of Functional Capacity: Validity, Intra- and Inter-rater Reliability

Defining “Arts Participation” for Public Health Research

Competence and Stress of Medication Administration Practices for School Nurses in K-12 Taiwan Schools

Wounds resulting from non-malignant haematological disease: a case series