Jialing Bao, Yunlin Tang, Yebo Chen, Jiangyan Jin, Xue Wang, Guozhen An, Lu Cao, Huarui Zhang, Gong Cheng, Guoqing Pan, Zeyang Zhou

E. hellemSer/Thr protein phosphatase PP1 targets the DC MAPK pathway and impairs immune functions

  • Health, Toxicology and Mutagenesis
  • Plant Science
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Ecology
Save to CiteDrive Show PDF

Microsporidia are difficult to be completely eliminated once infected, and the persistence disrupts host cell functions. Here in this study, we aimed to elucidate the impairing effects and consequences of microsporidia on host DCs.Enterocytozoon hellem, one of the most commonly diagnosed zoonotic microsporidia species, was applied. In vivo models demonstrated thatE. hellem-infected mice were more susceptible to further pathogenic challenges, and DCs were identified as the most affected groups of cells. In vitro assays revealed thatE. helleminfection impaired DCs’ immune functions, reflected by down-regulated cytokine expressions, lower extent of maturation, phagocytosis ability, and antigen presentations.E. helleminfection also detained DCs’ potencies to prime and stimulate T cells; therefore, host immunities were disrupted. We found thatE. hellemSer/Thr protein phosphatase PP1 directly interacts with host p38α (MAPK14) to manipulate the p38α(MAPK14)/NFAT5 axis of the MAPK pathway. Our study is the first to elucidate the molecular mechanisms of the impairing effects of microsporidia on host DCs’ immune functions. The emergence of microsporidiosis may be of great threat to public health.

Need a simple solution for managing your BibTeX entries? Explore CiteDrive!

  • Web-based, modern reference management
  • Collaborate and share with fellow researchers
  • Integration with Overleaf
  • Comprehensive BibTeX/BibLaTeX support
  • Save articles and websites directly from your browser
  • Search for new articles from a database of tens of millions of references
Try out CiteDrive

More from our Archive