Isolation of Fidaxomicin and Shunt Metabolites from Actinoplanes deccanensis
Erik Jung, Maja Hunter, Andrea Dorst, Alexander Major, Tatjana Teofilovic, Rolf Müller, Karl Gademann- Inorganic Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Drug Discovery
- Biochemistry
- Catalysis
A protocol for the isolation of the antibiotic fidaxomicin (Fdx) from Actinoplanes deccanensis and the isolation of shunt metabolites from A. deccanensisfdxG2‐ is reported. We constructed the mutant strain A. deccanensisfdxG2‐ by genetic manipulation which enabled the isolation of shunt metabolites as useful starting points for semisynthetic analogues of Fdx. Furthermore, a synthetic protocol for the conversion of complex A. deccanensisfdxG2‐ extracts into the single compound FdxG2‐OH via methanolysis is presented. This synthetic procedure is complemented by images and practical notes. Full structure assignment is given in the SI and the characterization data files are published to aid experimentalists. The protocol is also suitable as an undergraduate laboratory project. We hope to facilitate research into new Fdx derivatives through the availability of this procedure.