Ranjan Devkota, Jonnell C. Small, Kaycee Carbone, Michael A. Glass, Amedeo Vetere, Bridget K. Wagner

KD025 is a casein kinase 2 inhibitor that protects against glucolipotoxicity in beta cells

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine
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Glucolipotoxicity (GLT), in which elevated levels of glucose and fatty acids have deleterious effects on β-cell biology, is thought to be one of the major contributors in progression of type 2 diabetes. In search of novel small molecules that protects β-cells against GLT, we previously discovered KD025, an inhibitor of Rho-associated coiled-coil containing kinase isoform 2 (ROCK2), as a GLT-protective compound in INS-1E cells and dissociated human islets. To further understand the mechanism of action of KD025, we found that pharmacological and genetic inhibition of ROCK2 was not responsible for the protective effects of KD025 against GLT. Instead, kinase profiling revealed that KD025 potently inhibits catalytic subunits of casein kinase 2 (CK2), a constitutively active serine/threonine kinase. We experimentally verified that the inhibition of one of the catalytic subunits of casein kinase 2, CK2A1, but not CK2A2, improved cell viability when challenged with GLT. We conclude that KD025 inhibits CK2 to protect β-cells from glucolipotoxicity.

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