DOI: 10.1093/bbb/zbad170 ISSN: 1347-6947

SELEX-discovered aptamer that inhibits cellular interleukin-17/interleukin-17 receptor interaction and antagonizes interleukin-17 signaling

Arifur Rahman Munshi, Tong Wang, Yukio Takamori, Takehiro Ando, Takumi Yokoyama, Daisuke Fuji, Zhehao Xu, Santhana Vedi, Mizuki Yamamoto, Keita Tsukamoto, Takashi Kawakami
  • Organic Chemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • General Medicine
  • Biochemistry
  • Analytical Chemistry
  • Biotechnology
Show PDF Cite

Abstract

This research is based on a systematic evolution of ligands by exponential enrichment (SELEX), also referred to as in vitro selection against the extracellular domain of human interleukin-17 receptor A (IL-17RA). Pull-down assay via quantitative polymerase chain reaction and chemiluminescence detection showed that the cloned RNA with the enriched sequence bound to human IL-17RA and inhibited the interaction between IL-17RA and human interleukin-17A (IL-17A). We also revealed that the newly discovered IL-17RA-binding RNA aptamer bound to cellular IL-17RA, inhibited the cellular IL-17RA/IL-17A interaction, and antagonized cellular IL-17A signaling.

More from our Archive