DOI: 10.56530/lcgc.na.vr7280f7 ISSN:
Strongly Adsorbing Analytes: What, Why, and How to Fix It
Authors not available- Analytical Chemistry
In many applications of liquid chromatography (LC), excellent peak shapes are observed with simple, easy-to-use mobile phases and operating conditions. However, some specific combinations of analyte and LC column chemistry can lead to terrible peak shapes and even total adsorption of analytes to components of the LC system such that no peaks are observed at all. In this installment of “LC Troubleshooting”, I discuss two specific cases where an understanding of specific interactions between particular analyte functional groups and LC instruments or column materials is critical to achieving successful separations.