The Association between Histidine-Rich Glycoprotein rs10770 Genotype and Recurrent Miscarriage in Iranian Women

Purpose. Recurrent miscarriage (RM) is a significant reproductive concern affecting numerous women globally. Genetic factors are believed to play a crucial role in RM, making the histidine-rich glycoprotein (HRG) gene, a topic of interest due to its potential involvement in angiogenesis. This study is aimed at investigating the association between the HRG rs10770 genotype and RM. Method. Blood samples were collected from a total of 200 women at the beginning of the study. Subsequently, a comparative analysis was conducted between the blood samples of 100 women with a history of RM (case group) and the blood samples of another 100 healthy women (control group). HRG rs10770 genotyping was performed through polymerase chain reaction restriction-fragment length polymorphism (PCR-RFLP), followed by statistical analysis to evaluate the relationship between HRG rs10770 genotype and RM. Results. The results indicated a significant statistical difference between the C/C genotype ($\text{OR}=3.32$, CI: 1.22-9.04, $p=0.01$) and the C/T genotype ($\text{OR}=1.24$, CI: 0.67-2.30, $p=0.47$) in both the case and control groups. Additionally, a significant correlation was observed in the C allelic frequency among RM participants compared to the control group ($\text{OR}=1.65$, CI: 1.06-2.58, $p=0.02$). Conclusion. The study highlights the importance of HRG rs10770 in understanding RM, shedding light on its implications for reproductive health. Furthermore, it became evident that women carrying the homozygous C/C genotype exhibited increased susceptibility to the risk of RM.