DOI: 10.1007/bf02531316 ISSN:
Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots
George Rouser, Sidney Fleischer, Akira Yamamoto- Cell Biology
- Organic Chemistry
- Biochemistry
Abstract
Separation of polar lipids by two‐dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible procedure for determination of phospholipids by phosphorus analysis of spots are described. Values obtained for brain and mitochondrial inner membrane phospholipids are presented.